基于SPE与SPATT的水体中麻痹性贝类毒素检测方法构建与应用   总被引：1，自引：0，他引：1  

张亚亚  闫国旺  吴海燕  谭志军  张志华  江涛  李玉 《海洋与湖沼》2020,51(2):298-306

为实现麻痹性贝类毒素(paralytic shellfish poisoning,PSP)的实时监控与提前预警,本研究构建了基于固相萃取技术(solid phase extraction,SPE)与固相吸附毒素跟踪技术(solid phase adsorption toxin tracking,SPATT)的水体中PSP检测方法,重点优化了吸附材料及前处理方法,评价了回收率、检出限等指标,并将方法应用于2019年春季秦皇岛山海关海域PSP消长过程的监测中,比较评估了两种方法的监控预警效果。结果表明:SPE方法选用ENVI-Carb 500mg/6mL固相萃取柱,过样体积为50mL,13种PSP组分的平均回收率为82.2%±10.0%、检出限为4.0-20.0ng/L;SPATT方法选用SP207大孔吸附树脂,洗脱时间为静置Id最佳,整体回收率约为9.2%;在实际应用中,结合产毒藻密度及贻贝富集毒素含量的变化,发现SPE方法的检测结果可实时表征海域PSP风险状况,对于贻贝中PSP的预警效果也显著优于SAPTT方法,后者不仅因监控方式相对滞后一个监测周期,且灵敏度及准确性均较差。对于秦皇岛海域,当SPE方法检测结果达到100ng STX eq/L时,该海域贻贝中PSP残留将具有潜在的食用安全风险,跟踪过程表明这一阈值可提前两周预警贻贝富集毒素含量超出我国限量标准(800μg STX eq/kg),这对于强化风险警示并制定防范措施具有积极作用。  相似文献   

黄条(鰤)(Seriola aureovittata)MyHC基因克隆及其在早期发育阶段表达研究     

史宝  孙冉冉  柳学周  徐永江  姜燕  王滨  张正荣 《海洋与湖沼》2020,51(2):422-432

为解析肌球蛋白重链(MyHC)基因在黄条鰤(Seriola aureovittata)生长发育过程中作用机制,本研究采用RACE技术,克隆了黄条鰤MyHC基因全长cDNA序列,采用荧光定量PCR(qRT-PCR)方法对MyHC在黄条鰤不同组织、胚胎发育过程以及仔稚幼鱼发育过程中的表达特征进行了研究。结果表明:黄条鰤MyHC基因全长为6143bp,开放阅读框为5811bp,编码了1936个氨基酸,由3个保守结构域组成即MYSc-classⅡ、Myosin taill和SH3;系统进化树分析显示黄条鰤MyHC与高体鰤进化关系最近。qRT-PCR分析发现黄条鰤MyHC在各组织中均有表达,但在肌肉中表达量最高(P0.05);随着黄条鰤胚胎发育的进行,MyHC在16细胞期之前表达量较高,在胚体下包2/3时期表达量显著升高,且在孵化期达到峰值(P0.05:);在仔稚幼鱼发育阶段,MyHC在孵化后20d后表达量显著升高,30d表达水平达到峰值(P0.05),随后的35d到40d表达水平略有下降但仍保持较高表达趋势,黄条鰤MyHC表达具有发育阶段表达的特异性。MyHC表达特征揭示其参与了调控黄条鰤的早期生长发育。  相似文献   

守初心耕海探洋七十载担使命披风踏浪砥砺行——中国科学院海洋研究所70年发展纪实   总被引：1，自引：0，他引：1  

王凡  孙松 《海洋与湖沼》2020,51(4):664-672

中国科学院海洋研究所是新中国第一个专门从事海洋科学研究的国立科研机构,她的70年光辉历程是我国海洋科技事业从无到有、从弱到强的发展缩影,是新中国海洋科学调查研究自主创新发展历程的真实写照。本文简要介绍中国科学院海洋研究所的主要发展历程,回顾70年来取得的主要科技成就,并围绕新形势下建设海洋大科学研究中心等改革发展举措,讨论和展望研究所的未来发展。  相似文献   

hedgehog基因在笠贝(Lottia goshimai)早期发育中的表达模式研究     

杨伟红  郇聘  刘保忠 《海洋与湖沼》2020,51(4):960-965

Hedgehog信号通路参与动物神经系统发育、分节等诸多发育过程,研究该信号通路对理解动物的发育与演化机制有重要意义。对软体动物而言,目前Hedgehog信号通路的研究较少。本文克隆了腹足纲软体动物笠贝的hedgehog基因,利用整装原位杂交技术研究了其在胚胎发育过程中的时空表达规律;并与重要发育基因brachyury及soxb的表达模式进行了比较。结果表明,笠贝hedgehog基因与神经外胚层及中/内胚层相关,可能调控了幼虫神经、肌肉及消化道的分化。通过比较原肠胚及担轮幼虫不同阶段的基因表达模式,研究了hedgehog基因相关的组织和细胞随个体发育进程的动态变化。这些结果为理解软体动物形态发生和重要器官的分化机制提供了基础。  相似文献   

The potential of contribution of mangrove-derived organic matter in intertidal sediments as a proxy of mangrove development in the northern Beibu Gulf     

Jun Zhang  Xianwei Meng  Peng Xia  Xiangqin Wang  Shan Gao 《海洋学报(英文版)》2020,39(12):21-29

Located between terrestrial and marine ecosystems, mangrove forests are sensitive to changes in climate. The responses of mangrove ecosystems to climate change in the future can be understood by reconstructing past mangrove dynamics using proxies preserved in the intertidal sediments. Considering the complexity of the proxies commonly used, it is necessary to develop a relatively simple, inexpensive proxy. In this study, available chemical tracers (δ13C_org and C:N) of the four cores (YLW02, YLW03, O18, and Q37) from the intertidal zone of the northern Beibu Gulf (NBG) and a three-end-member (mangrove, sea grass, and suspended particulate matter) model was utilized to determine the contribution of mangrove-derived organic matter (CMOM) in carbonate-free sediments. Compared with the summed concentration of mangrove pollen (SCMP), a significant positive correlation between CMOM and SCMP is displayed. The calculated CMOM for an additional 210Pb-dated sediment core from the Yingluo Bay, NBG (YLW01) clearly indicates a mangrove development going through degradation, flourishing, relative degradation, and relative flourishing, which are separately in correspondence with the lowest, highest, lower, and higher air temperature and rainfall in the time intervals of 1890–1918 AD, 1919–1956 AD, 1957–1990 AD, and 1991–2010 AD. This suggests that CMOM preserved in intertidal sediments has a potential to reconstruct historical mangrove development in high resolution, at the very least, along the coasts of the NBG.  相似文献   

“数字海底”--海底数据集成管理与可视化研究     

肖鹏  王悦东  李安龙 《海洋信息》2020,35(1):40-45,51

针对目前"数字海底"建设中存在海底调查数据集成管理与三维可视化表达不足等问题,本文采用GIS技术,以黄河水下三角洲埕北海域为研究对象,利用地理空间数据集成理论与三维建模方法,建立了埕北海域三维海底空间数据库,实现了研究区域地形数据、地层数据、钻孔数据、表层沉积物数据的有效组织管理与可视化,并在此基础上,采用ArcGIS Engine 10.0,结合.NET平台,在Visual Studio 2010开发环境下,利用C#语言进行了二次开发,实现了基于C/S (Client/Server)架构的三维海底虚拟仿真系统的开发,设计了一套面向埕北海域的三维海底虚拟仿真原型系统。  相似文献   

粤港澳大湾区海洋经济创新发展研究          下载免费PDF全文

谢凡  张锐 《海洋开发与管理》2020,37(6):65-69

海洋经济创新发展对粤港澳大湾区创新驱动、打造具有国际竞争力的科技创新中心具有重要意义。文章介绍粤港澳大湾区当前的海洋经济创新发展情况及其优势和不足，从构建大湾区海洋创新发展产业链、驱动陆海科技创新一体化、完善科技创新驱动体制机制和推动科技金融服务蓝色经济发展等方面，提出粤港澳大湾区海洋经济创新发展的突破要点和路径。  相似文献   

The Digital Belt and Road program in support of regional sustainability     

Huadong Guo  Yubao Qiu  Massimo Menenti  Fang Chen  Paul F. Uhlir 《International Journal of Digital Earth》2018,11(7):657-669

The Belt and Road initiative has a significant focus on infrastructure, trade, and economic development across a vast region, and it also provides significant opportunities for sustainable development. The combined pressure of climate variability, intensified use of resources, and the fragility of ecosystems make it very challenging, however, to achieve future sustainability. To develop the path in a sustainable way, it is important to have a comprehensive understanding of these issues across nations and evaluate them in a scientific and well-informed approach. In this context, the Digital Belt and Road (DBAR) program was initiated as an international venture to share expertise, knowledge, technologies, and data to demonstrate the role of Earth observation science and technology and big Earth data applications to support large-scale development. In this paper, we identify pressing challenges, present the research priorities and foci of the DBAR program, and propose solutions where big Earth data can make significant contributions. This paper calls for further joint actions and collaboration to build a digital silk road in support of sustainable development at national, regional and global levels.  相似文献   

GIS professional development for teachers: lessons learned from high-needs schools     

Jerry T. Mitchell  George Roy  Stephannie Fritch  Brandy Wood 《制图学和地理信息科学》2018,45(4):292-304

University faculty partners from the Departments of Geography and Instruction and Teacher Education at a large, public university collaborated with K-12 teachers and the leadership of a rural school district in order to investigate the crosscutting content of science, mathematics, and geography through the integration of web-based GIS technologies. The project explored the critical connections among technology, pedagogy, and content with a particular emphasis on developing technology-enhanced, inquiry-based lessons in which the teachers and their students used GIS technologies to analyze, visualize, and present data in real-world contexts. The findings highlight the importance of well-structured professional development that builds community, integrates diverse content and pedagogical expertise, provides feedback and coaching, and is of sufficient duration to effect change.  相似文献   

FY-3E: The First Operational Meteorological Satellite Mission in an Early Morning Orbit     

Peng ZHANG  Xiuqing HU  Qifeng LU  Aijun ZHU  Manyun LIN  Ling SUN  Lin CHEN  Na XU 《大气科学进展》2022,39(1):1-1

Fengyun-3 E(FY-3E),the world’s first early-morning-orbit meteorological satellite for civil use,was launched successfully at the Jiuquan Satellite Launch Center on 5 July 2021.The FY-3E satellite will fill the vacancy of the global early-morning-orbit satellite observation,working together with the FY-3C and FY-3D satellites to achieve the data coverage of early morning,morning,and afternoon orbits.The combination of these three satellites will provide global data coverage for numerical weather prediction(NWP)at 6-hour intervals,effectively improving the accuracy and time efficiency of global NWP,which is of great significance to perfect the global earth observing system.In this article,the background and meteorological requirements for the early-morning-orbit satellite are reviewed,and the specifications of the FY-3E satellite,as well as the characteristics of the onboard instrumentation for earth observations,are also introduced.In addition,the ground segment and the retrieved geophysical products are also presented.It is believed that the NWP communities will significantly benefit from an optimal temporal distribution of observations provided by the early morning,mid-morning,and afternoon satellite missions.Further benefits are expected in numerous applications such as the monitoring of severe weather/climate events,the development of improved sampling designs of the diurnal cycle for accurate climate data records,more efficient monitoring of air quality by thermal infrared remote sensing,and the quasicontinuous monitoring of the sun for space weather and climate.  相似文献